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. 2018 Mar 13;6(1):4-9.
doi: 10.1055/s-0038-1636948. eCollection 2018 Mar.

Migration of Mesenchymal Stem Cells of Bursal Tissue after Rotator Cuff Repair in Rats

Elem Safi  1 , Andreas Ficklscherer  1 , Maryna Bondarava  1 , Oliver Betz  1 , Anja Zhang  1 , Volkmar Jansson  1 , Peter E Müller  1
Affiliations

Migration of Mesenchymal Stem Cells of Bursal Tissue after Rotator Cuff Repair in Rats

Elem Safi et al. Joints. .

Abstract

Purpose The purpose of this study is to verify migration of mesenchymal stem cells of bursal tissue into the healing site after rotator cuff repair in rats. Methods Fischer rats and green fluorescent protein (GFP)-transgenic rats were used. Bursal tissue from GFP rats was isolated and transplanted into tendon repair sites in Fischer rats. We examined the histology of the rotator cuff and the proportion of GFP-positive cells in the repaired rotator cuff 1, 3, and 6 weeks after surgery. Results Cell migration was observed during the third and sixth week after surgery. We also found mesenchymal stem cells and formed bursal cluster patterns in the repaired rotator cuff tendons. Conclusion Mesenchymal stem cells migrated from bursal tissue and infiltrated the repaired rotator cuff tendons. Clinical Relevance Mesenchymal stem cells from bursal tissue can contribute to the healing progress of the repaired rotator cuff.

Keywords: bursal tissue; cell migration; mesenchymal stem cells; rats; rotator cuff.

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Conflict of interest statement

Conflict of Interest None.

Figures

Fig. 1
Fig. 1
Bursa implantation after 1 week. On the left side, the transition border between bursal and surrounding tissue is visible. Bursal tissue of green fluorescent protein rat, consisting of fat vacuole and connective tissue, sized as compact tissue. A migration of mesenchymal stem cells is not observed.
Fig. 2
Fig. 2
Three weeks after bursa implantation, transition border between bursal tissue and tendon tissue of the rat is visible. Isolated bursal clusters are demonstrated which now are partly interspersed and not sized as compacted tissue (left picture). Golden cells originate from F344 rat compared with green cells from bursal tissue (GFP rat) at the transition between bursal and tendon/muscle tissue ( A–D : magnification x100).
Fig. 3
Fig. 3
Six weeks after surgical procedure, the transition borders between bursal tissue and tendon tissue were compounded. The bursal tissue seemed to adhere with surrounding tissue to form a bursal–tendon–muscle unit ( A and B : magnification x100; C and D : magnification x200).
Fig. 4
Fig. 4
Evidence of mesenchymal stem cells (MSCs) by staining due to immunostaining (CD73). ( A ) Proof of vascular tissue with surrounding MSCs. ( B ) Nuclei-staining in blue. ( C ) Combination and overlayed pattern of nuclei and MSCs around vascular tissue.
See this image and copyright information in PMC

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