Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2018 Apr 11:13:9.
doi: 10.1186/s40793-018-0309-z. eCollection 2018.

High quality draft genome sequence of Mycoplasma testudineum strain BH29T, isolated from the respiratory tract of a desert tortoise

Chava L Weitzman  1 , Richard L Tillett  2 , Franziska C Sandmeier  3 , C Richard Tracy  1 , David Alvarez-Ponce  1
Affiliations
Case Reports

High quality draft genome sequence of Mycoplasma testudineum strain BH29T, isolated from the respiratory tract of a desert tortoise

Chava L Weitzman et al. Stand Genomic Sci. .

Abstract

Mycoplasma testudineum is one of the pathogens that can cause upper respiratory tract disease in desert tortoises, Gopherus agassizii. We sequenced the genome of M. testudineum BH29T (ATCC 700618T = MCCM 03231T), isolated from the upper respiratory tract of a Mojave desert tortoise with upper respiratory tract disease. The sequenced draft genome, organized in 25 scaffolds, has a length of 960,895 bp and a G + C content of 27.54%. A total of 788 protein-coding sequences, six pseudogenes and 35 RNA genes were identified. The potential presence of cytadhesin-encoding genes is investigated. This genome will enable comparative genomic studies to help understand the molecular bases of the pathogenicity of this and other Mycoplasma species.

Keywords: Desert tortoise; Gopherus; Mycoplasma testudineum; URTD; Upper respiratory tract disease.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Transmission electron micrograph of thin section of Mycoplasma testudineum strain BH29T. Image from ref. [3], reproduced with permission from the publisher
Fig. 2
Fig. 2
Phylogenetic tree of the Mycoplasma genus based on 16S rRNA gene sequences showing the phylogenetic position of M. testudineum BH29T (●). All 16S sequences from the Mycoplasma genus were obtained from the SILVA database [17]. Only sequences in the ‘The All-Species Living Tree’ Project (LTP), release 128, were retained. This dataset only contains sequences from type strains, designated with a superscripted “T”. Clostridium botulinum strain ATCC 25763 was also included in the dataset as outgroup. Sequences were aligned using MUSCLE 3.8.31 [18]. A phylogenetic tree was obtained using the maximum likelihood method implemented in MEGA7 [19], with 1000 bootstrap replicates. Species with available genomes at the NCBI Genomes database or the Genomes Online Database are represented in bold face. GenBank accession numbers are shown in parentheses. Bootstrap support values above 50% are represented. The scale bar represents a divergence of 0.05 nucleotide substitutions per nucleotide position
Fig. 3
Fig. 3
Comparison of the 16S rRNA gene sequences generated by Brown et al. [3], by Volokhov et al. [24], and in our study. All three sequences correspond to M. testudineum BH29T. Asterisks represent identical sites
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Brown DR. Mycoplasmosis and immunity of fish and reptiles. Front Biosci. 2002;7:d1338–d1346. doi: 10.2741/A844. - DOI - PubMed
    1. Brown MB, Schumacher IM, Klein PA, Harris K, Correll T, Jacobson ER. Mycoplasma agassizii causes upper respiratory tract disease in the desert tortoise. Infect Immun. 1994;62(10):4580–4586. - PMC - PubMed
    1. Brown D, Merritt J, Jacobson E, Klein P, Tully J, Brown M. Mycoplasma testudineum sp. nov., from a desert tortoise (Gopherus agassizii) with upper respiratory tract disease. Int J Syst Evol Microbiol. 2004;54(5):1527–1529. doi: 10.1099/ijs.0.63072-0. - DOI - PubMed
    1. Jacobson ER, Berry KH. Mycoplasma testudineum in free-ranging desert tortoises, Gopherus agassizii. J Wildl Dis. 2012;48(4):1063–1068. doi: 10.7589/2011-09-256. - DOI - PubMed
    1. Brown MB, McLaughlin GS, Klein PA, Crenshaw BC, Schumacher IM, Brown DR, Jacobson ER. Upper respiratory tract disease in the gopher tortoise is caused by Mycoplasma agassizii. J Clin Microbiol. 1999;37:2262–2269. - PMC - PubMed

Publication types

LinkOut - more resources