The ATP-driven primary Na+ pump in subcellular vesicles of Vibrio alginolyticus
- PMID: 2968282
- DOI: 10.1016/0014-5793(88)80459-9
The ATP-driven primary Na+ pump in subcellular vesicles of Vibrio alginolyticus
Abstract
Subcellular vesicles of Vibrio alginolyticus hydrolyze ATP and accumulate Na+ in an ATP-dependent fashion. The Na+ uptake is (i) strongly stimulated by delta psi-discharging agents, i.e., the protonophorous uncoupler CCCP or valinomycin + K+ and (ii) arrested by DCCD at a concentration strongly inhibiting ATP hydrolysis. Lower concentrations of DCCD stimulate the Na+ accumulation supported by ATP hydrolysis as well as by NADH oxidation. It is concluded that there is an electrogenic DCCD-sensitive Na+-ATPase in the cytoplasmic membrane of V. alginolyticus.
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