doi: 10.1126/science.2968651.
A transcriptional enhancer 3' of C beta 2 in the T cell receptor beta locus
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- PMID: 2968651
- DOI: 10.1126/science.2968651
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A transcriptional enhancer 3' of C beta 2 in the T cell receptor beta locus
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Abstract
Run-on transcription experiments were used to demonstrate that transcription of T cell receptor beta chain V genes is activated by DNA rearrangement, in a manner similar to immunoglobulin genes. A transcriptional enhancer likely to be involved in this activation has been identified. A 25-kilobase region from J beta 1 to V beta 14 was tested for enhancer activity by transient transfections, and an enhancer was found 7.5 kilobases 3' of C beta 2. The beta enhancer has low activity relative to the simian virus 40 viral enhancer, does not display a preference for V beta promoters, has a T cell-specific activity, and binds two purified immunoglobulin heavy chain enhancer factors.
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