Polyelectrolyte complex of Aloe vera, chitosan, and alginate produced fibroblast and lymphocyte viabilities and migration

Abstract

Chitosan, sodium alginate and gel of Aloe vera (Aloe barbadensis Miller) were employed for the preparation of polyelectrolyte complexes at pH 4 and 6. FT-IR spectroscopy analysis showed evidence on complexes formation and incorporation of the Aloe vera gel. The ζ potential determination of the polyelectrolyte complexes revealed the presence of surface charges in the range of -20 to -24 mV, which results in stable systems. The dynamic moduli exhibited a high dependence on angular frequency, which is commonly found in solutions of macromolecules. The materials showed human fibroblast and lymphocyte viabilities up to 90% in agreement with null cytotoxicity. The polyelectrolyte complexes at pH 6 with Ca²⁺ were stable, showed high water absorption, satisfactory morphology, pore size and rigidity, characteristics that allowed significant human fibroblast migration in wound closure in vitro assays.

Keywords: (3-(4,5-Dimethyl-thiazol-2-yl)-2-5-diphenyltetrazolium bromide (PubChem CID: 64965); Aloe vera; Calcein-AM (PubChem CID:4126474); Calcofluor white (PubChem CID: 6108780); Cell viability; Chitosan; Chitosan (PubChem CID:71853); Dimethyl sulfoxide (PubChem CID: 679); Ethidium homodimer (PubChem CID: 12328897); Fibroblast; Isopropyl alcohol (PubChem CID: 3776); Lymphocyte; Polyelectrolyte complex; Sodium alginate (PubChem CID: 91666323).