Microbiological diagnosis of bacteraemia and fungaemia: Blood cultures and molecular methods

Abstract

Objetive: In this review we try to update the new procedures applicable in the microbiological diagnosis of bacteriemia and fungemias.

Method: Review of scientific literature.

Results and conclusions: After defining the process and indicating its fundamental principles, the main biomarkers used in clinical practice are reviewed. Subsequently, the particularities of the pre-analytical phase (collection and transport of samples) are highlighted and the steps to follow for the microbiological identification by classical methods are detailed, based on the culture of the blood samples. In the following section, we review the diagnostic methods not culture based, including those that detect the presence of the genome of the microorganism and those based on the study of proteome by mass spectrometry. The last section describes the procedures more frecuently used for the study of antibiotic susceptibility, both by phenotypic and genotypic methods.

Keywords: Bacteraemia; Bacteriemia; Blood cultures; Diagnóstico microbiológico; Fungaemia; Fungemia; Hemocultivos; Microbiological diagnosis.