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. 2018 Feb 27:23:77-82.
doi: 10.1016/j.nmni.2018.02.010. eCollection 2018 May.

Genome sequence and description of Paenibacillus ihuae strain GD6 sp. nov., isolated from the stool of a 62-year-old Frenchman

C Al-Bayssari  1 , G Dubourg  1 , T Cimmino  1 , L Okdah  1 , N Mathlouthi  1 , T T Nguyen  1 , C Andrieu  1 , J Rathored  1 , P-E Fournier  1 , D Raoult  1 , J-M Rolain  1
Affiliations

Genome sequence and description of Paenibacillus ihuae strain GD6 sp. nov., isolated from the stool of a 62-year-old Frenchman

C Al-Bayssari et al. New Microbes New Infect. .

Abstract

Paenibacillus ihuae strain GD6 (=CSUR P892 = DSMZ 45751T) is the new type strain collected from the stool of a 69-year-old Frenchman admitted to an intensive care unit and receiving a 10-day course of imipenem at the time of stool collection. This is a Gram-positive, facultative anaerobic, rod-shaped bacterium. We describe here the features of this organism, together with its complete genome sequence and annotation. The genome size is 6 719 043 bp with 49.6% G+C content and contains 6211 protein-coding and 65 sRNA genes, including four 5S rRNA genes, one 16S rRNA gene and one 23S rRNA gene.

Keywords: Culturomics; Paenibacillus ihuae; taxonogenomics.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree highlighting position of Paenibacillus ihuae strain GD6 16S rRNA gene sequence relative to other type strains within Paenibacillus genus. Sequences were aligned using MUSCLE, and phylogenetic inferences were obtained using maximum-likelihood method within MEGA6. Numbers at nodes are percentages of bootstrap values obtained by repeating analysis to generate majority consensus tree 1000 times.
Fig. 2
Fig. 2
Transmission electron microscopy of Paenibacillus ihuae strain GD6 using TechnaiG2 Cryo (FEI Company, Limeil-Brevannes, France) at operating voltage of 200 keV. Scale bar = 500 nm.
Fig. 3
Fig. 3
Phylogenetic of cluster representative of nonribosomal peptide synthase Paenibacillus ihuae strain GD6. Comparison of nonribosomal peptide synthetase (NRPS) of Paenibacillus sp. GD6 with closer cluster in other species. Percentages of identity are indicated for homologs found in cluster of WP 054942807.1 nonribosomal peptide synthetase of Paenibacillus sp. GD6 and closer cluster in other species.
See this image and copyright information in PMC

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