Pannonibacter carbonis sp. nov., isolated from coal mine water

Abstract

Two bacterial strains were isolated from coal mine water in China. Isolates were facultatively anaerobic, Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum, and they did not produce bacteriochlorophyll α. Cells grew in tryptic soy broth with 0-5.5 % (w/v) NaCl, at 4-55 °C and pH 3.5-10.5. Isolates were positive for catalase, oxidase, urease, Voges-Proskauer test, gelatin hydrolysis and H2S production. Analysis of 16S rRNA gene sequences indicated that the closest relatives of strains Q4.6^T and Q2.11 were the type strains Labrenzia suaedae DSM 22153^T (97.4 %), Pannonibacter phragmitetus DSM 14782^T (96.9 and 97.0 %) and Pannonibacter indicus DSM 23407^T (96.8 %). The genomic average nucleotide identity (ANI) value for Q4.6^T and Q2.11 was 100 %; however, this value was less than 77.7 % for the type strains P. phragmitetus and P. indicus, and less than 74.0 % for the type strain L. suaedae. The cellular fatty acid profile of strains Q4.6^T and Q2.11 consisted primarily of C18 : 1ω7c. The principal quinone of the isolates was Q-10. The polar lipid profile consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidyl choline. On the basis of phylogenetic analysis, genomic ANI analysis, DNA-DNA hybridization results, as well as phenotypic and chemotaxonomic data, strains Q4.6^T and Q2.11 are assigned as a novel species within the genus Pannonibacter. The type strain is Pannonibacter carbonis Q4.6^T (=CGMCC 1.15703^T=KCTC 52466^T).

Keywords: Pannonibacter; Pannonibacter carbonis sp. nov; new species.