Publisher Correction: Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells

Abstract

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Figure 1

The effect of pharmacological inhibitors on the engagement of liposomes delivered luc-siRNA-molecular beacon (MB) with target mRNA in A549-luc cells. Luc-siRNA-liposomes prepared at an N/P ratio 3.125:1, DC-Chol:DOPE ratio of 1:1, applied at 1 μg of luc-siRNA per well and at 1 mM total lipid content. Fluorescence from flow cytometry experiments expressed relative to the control (cells without inhibitors representing 100%); data represent the mean ± SD (N = 2, n = 4), **** indicate a significant difference between the results (p < 0.0001) and ns indicates the difference is a non-statistically significant (p > 0.05) compared to the control. Confocal microscopy micrographs show cells treated with ‘luc-siRNA based Molecular Beacon’ (MB) liposomes in A549-luc cells in the absence or presence of certain inhibitors. Engaged ‘Luc-siRNA Molecular Beacon’ siRNA appears green, whereas nuclei appear blue, staining with DRAQ5.