Next-generation diagnostics with CRISPR

Affiliations

¹ Critical Care Medicine Department, NIH Clinical Center, and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA. chertowd@cc.nih.gov.

Comment

Daniel S Chertow. Science. 2018.

. 2018 Apr 27;360(6387):381-382.

doi: 10.1126/science.aat4982.

¹ Critical Care Medicine Department, NIH Clinical Center, and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA. chertowd@cc.nih.gov.

No abstract available

Comment on

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.
Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Gootenberg JS, et al. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. Science. 2018. PMID: 29449508 Free PMC article.
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.
Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA. Chen JS, et al. Science. 2018 Apr 27;360(6387):436-439. doi: 10.1126/science.aar6245. Epub 2018 Feb 15. Science. 2018. PMID: 29449511 Free PMC article.
Field-deployable viral diagnostics using CRISPR-Cas13.
Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC. Myhrvold C, et al. Science. 2018 Apr 27;360(6387):444-448. doi: 10.1126/science.aas8836. Science. 2018. PMID: 29700266 Free PMC article.