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. 2018 Apr 30;13(4):e0196572.
doi: 10.1371/journal.pone.0196572. eCollection 2018.

Genetic profiling of young and aged endothelial progenitor cells in hypoxia

Affiliations

Genetic profiling of young and aged endothelial progenitor cells in hypoxia

Tzu-Wei Wu et al. PLoS One. .

Abstract

Age is a major risk factor for diseases caused by ischemic hypoxia, such as stroke and coronary artery disease. Endothelial progenitor cells (EPCs) are the major cells respond to ischemic hypoxia through angiogenesis and vascular remodeling. However, the effect of aging on EPCs and their responses to hypoxia are not well understood. CD34+ EPCs were isolated from healthy volunteers and aged by replicative senescence, which was to passage cells until their doubling time was twice as long as the original cells. Young and aged CD34+ EPCs were exposed to a hypoxic environment (1% oxygen for 48hrs) and their gene expression profiles were evaluated using gene expression array. Gene array results were confirmed using quantitative polymerase chain reaction, Western blotting, and BALB/c female athymic nude mice hindlimb ischemia model. We identified 115 differentially expressed genes in young CD34+ EPCs, 54 differentially expressed genes in aged CD34+ EPCs, and 25 common genes between normoxia and hypoxia groups. Among them, the expression of solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1) increased the most by hypoxia in young cells. Gene set enrichment analysis indicated the pathways affected by aging and hypoxia most, including genes "response to oxygen levels" in young EPCs and genes involved "chondroitin sulfate metabolic process" in aged cells. Our study results indicate the key factors that contribute to the effects of aging on response to hypoxia in CD34+ EPCs. With the potential applications of EPCs in cardiovascular and other diseases, our study also provides insight on the impact of ex vivo expansion might have on EPCs.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Isolation and aging of CD34+ EPC.
Isolated CD34+ EPC were subcultured in vitro and signs of senescence showed after around 10 passages. Replicative senescence resulted in (A) morphological changes (Bar = 10μm), (B) up-regulation of senescence marker (P < .01).
Fig 2
Fig 2. Determination of hypoxia time.
(A) VEGF Western blot time course showed that VEGF was upregulated within 24 hours and reached a plateau in 48 hours. N = 3; * P< .01; # P< .05 (B) VEGF upregulation was confirmed by expression microarray in 3 EPC clones.
Fig 3
Fig 3. Quantitative PCR for control genes.
(A) SLC2A1 was significantly up-regulated by hypoxia. No significant difference was found in 18S (B) and HPRT (C) expression. (D) ADM was significantly upregulated and (E) HMOX1 was downregulated by hypoxia which was consistent with microarray results. N = 6 * P < .05 compared to normoxia cells of the same passage. Y: young; A: aged; N: normoxia; H: hypoxia.
Fig 4
Fig 4. Validation of SLC2A1 expression in response to hypoxia.
(A) Western blots showing a significant rise of the SLC2A1 protein level in young EPCs. Representative Western blots and normalized quantification of band density were shown. * p < .05 compared to young CD34+ EPCs in normoxia, N = 3, Bars represent mean ± SEM. (B) Hypoxia-induced membrane translocation of SLC2A1 (Bar = 10mm). (C) EPCs (labeled with HNA) injected into ischemic hindlimb (Left) expressed more SLC2A1 that EPCs injected into the contralateral tissue (Right). Y: young; A: aged; N: normoxia; H: hypoxia; HNA: human nuclear antigen.

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