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. 2018 Apr 19:10:57.
doi: 10.1186/s13148-018-0489-9. eCollection 2018.

Fetal growth is associated with CpG methylation in the P2 promoter of the IGF1 gene

Catherine Le Stunff  1 , Anne-Laure Castell  2 , Nicolas Todd  1 , Clémence Mille  1 , Marie-Pierre Belot  1 , Nathalie Frament  1 , Sylvie Brailly-Tabard  3 , Alexandra Benachi  4 , Delphine Fradin  5 , Pierre Bougnères  1
Affiliations

Fetal growth is associated with CpG methylation in the P2 promoter of the IGF1 gene

Catherine Le Stunff et al. Clin Epigenetics. .

Erratum in

Abstract

Background: There are many reasons to think that epigenetics is a key determinant of fetal growth variability across the normal population. Since IGF1 and INS genes are major determinants of intrauterine growth, we examined the methylation of selected CpGs located in the regulatory region of these two genes.

Methods: Cord blood was sampled in 159 newborns born to mothers prospectively followed during their pregnancy. A 142-item questionnaire was filled by mothers at inclusion, during the last trimester of the pregnancy and at the delivery. The methylation of selected CpGs located in the promoters of the IGF1 and INS genes was measured in cord blood mononuclear cells collected at birth using bisulfite-PCR-pyrosequencing.

Results: Methylation at IGF1 CpG-137 correlated negatively with birth length (r = 0.27, P = 3.5 × 10-4). The same effect size was found after adjustment for maternal age, parity, and smoking: a 10% increase in CpG-137 methylation was associated with a decrease of length by 0.23 SDS.

Conclusion: The current results suggest that the methylation of IGF1 CpG-137 contributes to the individual variation of fetal growth by regulating IGF1 expression in fetal tissues.

Keywords: DNA methylation; Epigenetics; Growth; Newborn.

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Conflict of interest statement

We obtained informed consent from all participating mothers. All protocols were agreed by French ethic boards (CODECOH DC-2013-2017, CPP CO-14-001, CCTIRS no. 14-124bis, CNIL no. 914253).The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
CpG-137 methylation correlates negatively with birth length. The correlation between CpG-137 (%) and birth length (SDS) is described by the equation: Birth length = − 0.023 × [CpG-137 methylation] + 0.68 with r = 0.2 and P = 3.5 × 10−4
See this image and copyright information in PMC

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