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. 2018;8(1):53-58.
doi: 10.15171/bi.2018.07. Epub 2017 Oct 30.

Chemiluminescence based immunoassay for the detection of heroin and its metabolites

Smritee Singh  1 , Priya Mishra  1   2 , Ivneet Banga  1 , Avanish S Parmar  3 , Prem Prakash Tripathi  4 , Sonu Gandhi  1
Affiliations

Chemiluminescence based immunoassay for the detection of heroin and its metabolites

Smritee Singh et al. Bioimpacts. 2018.

Abstract

Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples. Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples. Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/ mL in spiked urine samples and limit of detection were 80, 95, 90, 75 pg/ mL. Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs.

Keywords: Antibody; Chemiluminescence immunoassay; Horseradish peroxidase; Metabolites; Monoacetyl morphine.

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