Viral proteins targeting host protein kinase R to evade an innate immune response: a mini review

Abstract

The innate immune system offers a first line of defense by neutralizing foreign pathogens such as bacteria, fungi, and viruses. These pathogens express molecules (RNA and proteins) that have discrete structures, known as the pathogen-associated molecular patterns that are recognized by a highly specialized class of host proteins called pattern recognition receptors to facilitate the host's immune response against infection. The RNA-dependent Protein Kinase R (PKR) is one of the host's pattern recognition receptors that is a key component of an innate immune system. PKR recognizes imperfectly double-stranded non-coding viral RNA molecules via its N-terminal double-stranded RNA binding motifs, undergoes phosphorylation of the C-terminal kinase domain, ultimately resulting in inhibition of viral protein translation by inhibiting the guanine nucleotide exchange activity of eukaryotic initiation factor 2α. Not surprisingly, viruses have evolved mechanisms by which viral non-coding RNA or protein molecules inhibit PKR's activation and/or its downstream activity to allow viral replication. In this review, we will highlight the role of viral proteins in inhibiting PKR's activity and summarize currently known mechanisms by which viral proteins execute such inhibitory activity.

Keywords: Double-stranded RNA binding motifs; HCV: hepatitis C virus; HSV-1: herpes simplex virus 1 HIV-1: human immunodeficiency virus -1; PKR: RNA-activated Protein Kinase; RNA-activated Protein Kinase; RVFV: Rift valley fever virus; TAR: transactivation response; UTR: untranslated region; VAI: adenovirus virus-associated RNA; adenovirus virus-associated RNA; dsRBMs: double-stranded RNA binding motifs; eIF2α: eukaryotic initiation factor 2α; host-viral interactions; innate immunity; viral infection.