Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 May 2;18(1):140.
doi: 10.1186/s12906-018-2207-8.

Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria

Hazir Rahman  1 , Ilyas Khan  2 , Anwar Hussain  3 , Abdelaaty Abdelaziz Shahat  4   5 , Abdul Tawab  6 , Muhammad Qasim  2 , Muhammad Adnan  7 , Mansour S Al-Said  4 , Riaz Ullah  4 , Shahid Niaz Khan  8
Affiliations

Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria

Hazir Rahman et al. BMC Complement Altern Med. .

Abstract

Background: Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant's therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil.

Methods: The present investigation analyzed the High-Pressure Liquid Chromatography (HPLC) fractions of Glycyrrhiza glabra (Aqueous, Chloroform, Ethanol and Hexane) against multidrug resistant human bacterial pathogens (Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa). All the fractions showed antibacterial activity, were subjected to LC MS/MS analysis for identification of bioactive compounds.

Results: Among total HPLC fractions of G. glabra (n = 20), three HPLC fractions showed potential activity against multidrug resistant (MDR) bacterial isolates. Fraction 1 (F1) of aqueous extracts, showed activity against A. baumannii (15 ± 0.5 mm). F4 from hexane extract of G. glabra showed activity against S. aureus (10 ± 0.2 mm). However, F2 from ethanol extract exhibited activity against S. aureus (10 ± 0.3 mm). These active fractions were further processed by LC MS/MS analysis for the identification of compounds. Ellagic acid was identified in the F1 of aqueous extract while 6-aldehydo-isoophiopogonone was present in F4 of hexane extract. Similarly, Liquirtigenin was identified in F2 of ethanol.

Conclusions: Glycyrrhiza glabra extracts HPLC fractions showed anti-MDR activity. Three bioactive compounds were identified in the study. 6-aldehydo-isoophiopogonone and Liquirtigenin were for the first time reported in G. glabra. Further characterization of the identified compounds will be helpful for possible therapeutic uses against infectious diseases caused by multidrug resistant bacteria.

Keywords: Anti-MDR activity; Ellagic acid 6-aldehydo-isoophiopogonone; Glycyrrhiza glabra; HPLC fractionation; Liquirtigenin.

PubMed Disclaimer

Conflict of interest statement

Ethics approval and consent to participate

A local ethics committee ruled that no formal ethics approval was required in this particular case.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
LC MS/MS Chromatogram of fraction 1 (F1) from aqueous extracts of Glycyrrhiza glabra showing Ellagic Acid
Fig. 2
Fig. 2
LC MS/MS Chromatogram of fraction 4 (F4) from hexane extracts of Glycyrrhiza glabra showing 6-aldehydo-isoophiopogonone
Fig. 3
Fig. 3
LC MS/MS Chromatogram of fraction 2 (F2) from ethanol extracts of Glycyrrhiza glabra showing Liquirtigenin
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci. 2008;4:839–843.
    1. Van-Wyk BE. A review of ethnobotanical research in southern Africa. S Afr J Bot. 2002;68:1–13. doi: 10.1016/S0254-6299(16)30447-1. - DOI
    1. Bhojane P, Damle S, Thite A, Dabholkar V. Anti-microbial effects of some leafy vegetables - a comparative analysis. Int Res J Biol Sci. 2014;3:26–32.
    1. Talib WH, Mahasneh AM. Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules. 2010;15:1811–1824. doi: 10.3390/molecules15031811. - DOI - PMC - PubMed
    1. Hayashi H, Hattori S, Inoue K, Khodzhimatov O, Ashurmetov O, Ito M, Honda G. Field survey of Glycyrrhiza plants in Central Asia (3). Chemical characterization of G. Glabra collected in Uzbekistan. Chem Pharm Bull. 2003;51(11):1338–1340. doi: 10.1248/cpb.51.1338. - DOI - PubMed

MeSH terms