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. 2018 Apr 18:9:494.
doi: 10.3389/fpls.2018.00494. eCollection 2018.

Surface Properties and Permeability to Calcium Chloride of Fagus sylvatica and Quercus petraea Leaves of Different Canopy Heights

Héctor A Bahamonde  1   2 , Luis Gil  3 , Victoria Fernández  3
Affiliations

Surface Properties and Permeability to Calcium Chloride of Fagus sylvatica and Quercus petraea Leaves of Different Canopy Heights

Héctor A Bahamonde et al. Front Plant Sci. .

Abstract

Plant surfaces have a considerable degree of chemical and physical variability also in relation to different environmental conditions, organs and state of development. The potential changes on plant surface properties in association with environmental variations have been little explored so far. Using two model tree species (i.e., Quercus petraea, sessile oak and Fagus sylvatica, beech) growing in 'Montejo de la Sierra Forest,' we examined various traits of the abaxial and adaxial surface of leaves of both species collected at a height of approximately 15 m (top canopy), versus 3.5-5.5 m for beech and sessile oak, lower canopy leaves. Leaf surface ultra-structure was analyzed by scanning and transmission electron microscopy, and the surface free energy and related parameter were estimated after measuring drops of 3 liquids with different degrees of polarity and apolarity. The permeability of the adaxial and abaxial surface of top and bottom canopy leaves to CaCl2 was estimated by depositing 2 drops of 3-4 μl per cm2 and comparing the concentration of Ca in leaf tissues 24 h after treatment, and also Ca and Cl concentrations in the washing liquid. Higher Ca concentrations were recorded after the application of CaCl2 drops onto the veins and adaxial blade of top canopy beech leaves, while no significant evidence for foliar Ca absorption was gained with sessile oak leaves. Surprisingly, high amounts of Cl were recovered after washing untreated, top canopy beach and sessile oak leaves with deionised water, a phenomenon which was not traced to occur on lower canopy leaves of both species. It is concluded that the surface of the two species analyzed is heterogeneous in nature and may have areas favoring the absorption of water and solutes as observed for the veins of beech leaves.

Keywords: Fagus sylvatica; Quercus petraea; cuticle; foliar absorption; plant surfaces; waxes; wettability.

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Figures

FIGURE 1
FIGURE 1
Deposition of 150 mM CaCl2 drops onto beech and sessile oak adaxial and abaxial plant surfaces using a micro-syringe to carry out a foliar absorption trial.
FIGURE 2
FIGURE 2
Cross-sections of beech (A,B) and sessile oak (C,D) leaves, collected from upper (A,C) or lower (B,D) tree canopy positions, stained with toluidine blue and observed by optical microscopy.
FIGURE 3
FIGURE 3
Electron micrographs of adaxial and abaxial beech leaf surfaces. SEM images correspond to: (A) adaxial surface of an upper canopy leaf, (B) abaxial upper canopy leaf, (E) adaxial surface of a lower canopy leaf, (F) abaxial surface of a lower canopy leaf. TEM micrographs correspond to: (C) adaxial epidermis of an upper canopy leaf, (D) abaxial epidermis of an upper canopy leaf, (G) adaxial epidermis on a lower canopy leaf, (H) abaxial epidermis of a lower canopy leaf.
FIGURE 4
FIGURE 4
Electron micrographs of adaxial and abaxial sessile oak leaf surfaces. SEM images correspond to: (A) adaxial surface of an upper canopy leaf, (B) abaxial upper canopy leaf, (E) adaxial surface of a lower canopy leaf, (F) abaxial surface of a lower canopy leaf. TEM micrographs correspond to: (C) adaxial epidermis of an upper canopy leaf, (D) abaxial epidermis of an upper canopy leaf, (G) adaxial epidermis on a lower canopy leaf, (H) abaxial epidermis of a lower canopy leaf.
See this image and copyright information in PMC

References

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