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. 2018 Nov;49 Suppl 1(Suppl 1):185-192.
doi: 10.1016/j.bjm.2018.04.004. Epub 2018 Apr 24.

First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation

Cristiane Bianchi Loureiro Dos Reis  1 , Liziane Maria Barassuol Morandini  2 , Caroline Borges Bevilacqua  1 , Fabricio Bublitz  2 , Gustavo Ugalde  3 , Marcio Antonio Mazutti  4 , Rodrigo Josemar Seminoti Jacques  5
Affiliations

First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation

Cristiane Bianchi Loureiro Dos Reis et al. Braz J Microbiol. 2018 Nov.

Abstract

Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used. The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose. The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry. F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation. The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose. The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.

Keywords: Giberella fujikuroi; NMR spectroscopy; Surface tension; Trehalolipids; α,β-Trehalose.

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Figures

Fig. 1
Fig. 1
Dendrogram of Fusarium fujikuroi (Gibberella fujikuroi) obtained from sequences of the elongation factor 1α (EF-1α). Maximum likelihood (ML) analysis was performed with 1000 replicates. Bootstrap values are in percentages. Neonectria radicicola was used as an outgroup.
Fig. 2
Fig. 2
Pareto plot with temperature (T), agitation (A) and incubation time (I), which demonstrated statistical significance (p < 0.1) in a central rotational compound design (CCRD) aimed at reducing the surface tension of the culture.
Fig. 3
Fig. 3
Contour plot of the reduction of surface tension of the growth medium by production of biosurfactants by Fusarium fujikuroi UFSM-BAS-01. (I) Incubation period; (A) agitation; (T) temperature.
Fig. 4
Fig. 4
Surface tension (ST) of distilled water with the addition of increasing amounts of the biosurfactant produced by Fusarium fujikuroi UFSM-BAS-01.
See this image and copyright information in PMC

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