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Review
. 2018 Mar 30;6(3):e1030.
doi: 10.1002/aps3.1030. eCollection 2018 Mar.

A guide to sequence your favorite plant genomes

Fay-Wei Li  1   2 , Alex Harkess  3
Affiliations
Review

A guide to sequence your favorite plant genomes

Fay-Wei Li et al. Appl Plant Sci. .

Abstract

With the rapid development of sequencing technology and the plummeting cost, assembling whole genomes from non-model plants will soon become routine for plant systematists and evolutionary biologists. Here we summarize and compare several of the latest genome sequencing and assembly approaches, offering a practical guide on how to approach a genome project. We also highlight certain precautions that need to be taken before investing time and money into a genome project.

Keywords: Hi‐C; Illumina; Nanopore; PacBio; genome assembly; optical mapping.

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Figures

Figure 1
Figure 1
An example flow cytometry result, using Nicotiana as the standard to infer the genome size of the fern Parahemionitis cordata.
Figure 2
Figure 2
Hypothetical k‐mer frequency plots, showing a low‐heterozygosity genome (A) and a highly heterozygous and repetitive genome (B).
See this image and copyright information in PMC

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