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. 2017 Nov;23(11):963-968.

[Expression characteristics of the USP24 gene in the mouse testis during spermatogenesis]

[Article in Chinese]
Qi-Yi Hu  1 , Qiong Deng  1 , Jian-Wen Zhang  1 , Fan Zhi  1 , Rui Sun  1 , Hui Liang  1
Affiliations
  • PMID: 29738159

[Expression characteristics of the USP24 gene in the mouse testis during spermatogenesis]

[Article in Chinese]
Qi-Yi Hu et al. Zhonghua Nan Ke Xue. 2017 Nov.

Abstract
in English, Chinese

Objective: To investigate the expression characteristics of the USP24 gene in the mouse testis and its role in spermatogenesis.

Methods: We examined the expression characteristics of USP24 in the testis tissues of wild-type mice at different postnatal weeks (PNW) and androgen receptor (AR)-knockout (ARKO) adult mice using real-time quantitative PCR and immunofluorescence, and detected the transcriptional activity of the USP24 promoter by dual-luciferase reporter gene assay.

Results: The expression of the USP24 gene was low in the testis tissue of the wild-type mice at PNW 1, increased dramatically at PNW 3 and stayed at a similar level till PNW 8. The USP24 protein was located mainly in the cytoplasm of Sertoli and spermatogenic cells. Compared with the wild-type, the adult ARKO mice showed a decreased expression of USP24 localized in the posterior head and mid-piece of the mature sperm in the testis. Dual-luciferase reporter gene assay showed that the transcriptional activity of the USP24 promoter was increased after testosterone stimulation.

Conclusions: The increased expression of the USP24 gene was associated with the initiation of sexual development, and the USP24 protein was expressed in the mature sperm of the mice. USP24 is an AR-target gene, which may be involved in the regulation of spermatogenesis in mice.

目的： 研究去泛素化酶24（USP24）基因在小鼠睾丸精子发生过程中的表达特征，初步探讨其在生精过程中的作用。方法： 通过实时荧光定量PCR（qPCR）和免疫荧光等方法检测USP24在不同周龄野生型小鼠睾丸组织以及成年雄激素受体敲除（ARKO）小鼠睾丸中的表达特征；采用双荧光素酶报告基因实验检测USP24启动子转录活性。结果： qPCR和免疫荧光结果表明，USP24基因在出生1周时表达水平较低，3周时急剧升高，随后维持在相似水平至第8周；USP24主要定位于支持细胞和生精细胞的细胞质；与野生型相比，USP24在ARKO 小鼠睾丸表达降低；性成熟小鼠睾丸中，USP24定位于成熟精子头部后端及中部。双荧光素酶报告基因实验结果显示，睾酮刺激后USP24启动子的转录活性升高。结论： USP24基因的表达水平的升高与小鼠的性发育相关，且其蛋白在小鼠成熟精子上表达。USP24是受雄激素受体(AR)调控的靶基因。USP24可能参与调控小鼠的精子发生过程。.

Keywords: mouse; spermatogenesis; testis; ubiquitin-specific protease 24.

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