Expression of adhesion molecules on granulocytes and monocytes following myocardial infarction in rats drinking white wine

Abstract

Neutrophils and monocytes through their CD15s, CD11b and CD44 adhesion molecules are implicated in the initiation and resolution of cardiac inflammation as well as in healing processes after the myocardial infarction (MI). The aim of this study was to determine the effect of white wine consumption on granulocyte and monocyte CD15s, CD11b, and CD44 expression 24h after the surgically inflicted MI. Granulocytes and monocytes were analyzed by flow cytometry, using whole blood of male Sprague-Dawley rats that consumed white wine for 4 weeks. This group was compared with water only drinking controls, sham animals (subject to surgery without myocardial infarction) and baseline group (intact animals that received no intervention prior to being sacrificed). Sham animals did not differ from baseline animals in CD11b+CD44+ percentage and CD44+ median fluorescence intensity. Wine drinking was associated with striking increase in CD44 expression on monocyte subpopulations. Its expression was three and fourfold increased on monocytes and large monocytes, respectively, relative to the water only drinking controls. Because of known role of CD44 on suppression of post-infarction inflammation, its upregulation on granulocytes and monocytes may significantly contribute to the microenvironment favourable for the cardiac regeneration.

Fig 1. Representative gates for granulocytes (E1), monocyte (E2) and large monocyte (E3) populations from baseline (BL) and control (C) group.

Population gates on dot plots a-BL and a-C could not be determined, so they were zoomed and dot plots set “b” was created. The subpopulation of large monocytes appears in control group of animals, subjected to myocardial infarction but not in baseline group.

Fig 2. Expression of CD44, CD11b and CD15s on A) large monocytes, B) monocytes and C) granulocytes.

Median fluorescence intensity (MFI) of each group is normalised to and presented as fold change from baseline. Data for each subpopulation set is represented in diagrams with median values marked. Mann Whitney test was used for data analyses. Representative fluorescence histograms for each experimental group are provided. *-P<0.05, ***-P<0.001.