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. 2018 May 10;19(5):1424.
doi: 10.3390/ijms19051424.

Hierarchical ZIF-8 toward Immobilizing Burkholderia cepacia Lipase for Application in Biodiesel Preparation

Miaad Adnan  1   2 , Kai Li  3 , Jianhua Wang  4 , Li Xu  5 , Yunjun Yan  6
Affiliations

Hierarchical ZIF-8 toward Immobilizing Burkholderia cepacia Lipase for Application in Biodiesel Preparation

Miaad Adnan et al. Int J Mol Sci. .

Abstract

A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent. Burkholderia cepacia lipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.

Keywords: Burkholderia cepacia lipase (BCL); adsorption method; biodiesel production; hexahedral ZIF-8; hierarchical ZIF-8; surfactant.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic model to illustrate (a) synthesis hexahedral zeolitic imidazolate framework (ZIF-8); (b) synthesis hierarchical ZIF-8 and immobilization Burkholderia cepacia lipase (BCL)-ZIF-8.
Figure 2
Figure 2
TEM images: (a) hierarchical ZIF-8; (b) hexahedral ZIF-8; (c) BCL-ZIF-8.
Figure 3
Figure 3
SEM images: (a) hierarchical ZIF-8; (b) hexahedral ZIF-8; (c) BCL-ZIF-8.
Figure 4
Figure 4
(a) Fourier transform infrared (FT-IR) spectra of samples of hierarchical ZIF-8; BCL and BCL-ZIF-8; (b) powder X-ray diffraction (PXRD) patterns of samples of hierarchical ZIF-8 and BCL-ZIF-8.
Figure 5
Figure 5
Nitrogen gas adsorption/desorption isotherms and pore size distributions for (a) hexahedral ZIF-8 and (b) hierarchical ZIF-8.
Figure 6
Figure 6
Influence of various immobilization conditions on immobilization efficiency and activity recovery. (a) Amounts of lipase; (b) adsorption time; (c) reaction temperature; (d) pH value.
Figure 7
Figure 7
Influences of reaction parameters on biodiesel production catalyzed by BCL-ZIF-8: (a) lipase dosage; (b) molar ratio of oil to ethanol; (c) water content; (d) reaction time; (e) reaction temperature.
Figure 8
Figure 8
Reuse the catalyzed BCL-ZIF-8 for biodiesel production.
See this image and copyright information in PMC

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