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. 2018 May 10;19(1):353.
doi: 10.1186/s12864-018-4762-2.

Global evolution and phylogeography of Brucella melitensis strains

Sergey V Pisarenko  1 , Dmitry A Kovalev  2 , Anna S Volynkina  2 , Dmitry G Ponomarenko  2 , Diana V Rusanova  2 , Nina V Zharinova  2 , Anna A Khachaturova  2 , Lyudmila E Tokareva  3 , Irina G Khvoynova  3 , Alexander N Kulichenko  2
Affiliations

Global evolution and phylogeography of Brucella melitensis strains

Sergey V Pisarenko et al. BMC Genomics. .

Abstract

Background: Brucellosis is a bacterial zoonotic disease. Annually in the world more than 500,000 new cases of brucellosis in humans are registered. In this study, we propose an evolutionary model of the historical distribution of B. melitensis based on the full-genomic SNP analysis of 98 strains.

Results: We performed an analysis of the SNP of the complete genomes of 98 B. melitensis strains isolated in different geographical regions of the world to obtain relevant information on the population structure, genetic diversity and the evolution history of the species. Using genomic sequences of 21 strains of B. melitensis isolated in Russia and WGS data from the NCBI database, it was possible to identify five main genotypes and 13 species genotypes for analysis. Data analysis based on the Bayesian Phylogenetics and Phylogeography method allowed to determine the regions of geographical origin and the expected pathways of distribution of the main lines (genotypes and subgenotypes) of the pathogen.

Conclusions: Within the framework of our study, the model of global evolution and phylogeography of B. melitensis strains isolated in various regions of the planet was proposed for the first time. The sets of unique specific SNPs described in our study, for all identified genotypes and subgenotypes, can be used to develop new bacterial typing and identification systems for B. melitensis.

Keywords: Brucella melitensis; Evolution; Phylogeography; Single nucleotide polymorphism; Whole genome sequencing.

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Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Figures

Fig. 1
Fig. 1
The Bayesian phylogenetic tree shows the evolutionary relationships of the 88 B. melitensis isolates (those for which a fixed isolation date was established). The timeline indicates the date of divergence of the branches of the tree, the values of < 0 correspond to the years BC, the ends of the branches of the tree - the isolation time of the strains. The tree nodes indicate the probabilities and Bayesian estimation of time of divergence. The color of the terminal branches of the tree corresponds to the region in which the isolates were isolated
Fig. 2
Fig. 2
Geographical distribution of isolates. The labels indicate the geographical regions in which the strains were isolated. The color of the label corresponds to the genotype that we determined for each isolate, the number inside the label corresponds to the number of strains isolated in each region
See this image and copyright information in PMC

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