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Review
. 2018 Oct:153:3-12.
doi: 10.1016/j.biochi.2018.05.004. Epub 2018 May 9.

Chromatography of oxysterols

Irundika H K Dias  1 , Steven R Wilson  2 , Hanne Roberg-Larsen  3
Affiliations
Review

Chromatography of oxysterols

Irundika H K Dias et al. Biochimie. 2018 Oct.

Abstract

Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

Keywords: Chromatography; Lipids; Oxysterols; Separation; Sterols.

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