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. 2018 Sep:63:1-4.
doi: 10.1016/j.meegid.2018.05.004. Epub 2018 May 8.

High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei

G Girault  1 , P Wattiau  2 , M Saqib  3 , B Martin  1 , F Vorimore  1 , H Singha  4 , M Engelsma  5 , H J Roest  5 , S Spicic  6 , R Grunow  7 , N Vicari  8 , S C J De Keersmaecker  9 , N H C Roosens  9 , M Fabbi  8 , B N Tripathi  4 , S Zientara  1 , N Madani  1 , K Laroucau  10
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High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei

G Girault et al. Infect Genet Evol. 2018 Sep.

Abstract

Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.

Keywords: Burkholderia mallei; Genotyping; HRM.

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