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. 2018 May 3;23(5):1071.
doi: 10.3390/molecules23051071.

Chromatogram-Bioactivity Correlation-Based Discovery and Identification of Three Bioactive Compounds Affecting Endothelial Function in Ginkgo Biloba Extract

Hong Liu  1 , Li-Ping Tan  2   3 , Xin Huang  4 , Yi-Qiu Liao  5 , Wei-Jian Zhang  6 , Pei-Bo Li  7 , Yong-Gang Wang  8 , Wei Peng  9 , Zhong Wu  10 , Wei-Wei Su  11 , Hong-Liang Yao  12
Affiliations

Chromatogram-Bioactivity Correlation-Based Discovery and Identification of Three Bioactive Compounds Affecting Endothelial Function in Ginkgo Biloba Extract

Hong Liu et al. Molecules. .

Abstract

Discovery and identification of three bioactive compounds affecting endothelial function in Ginkgo biloba Extract (GBE) based on chromatogram-bioactivity correlation analysis. Three portions were separated from GBE via D101 macroporous resin and then re-combined to prepare nine GBE samples. 21 compounds in GBE samples were identified through UFLC-DAD-Q-TOF-MS/MS. Correlation analysis between compounds differences and endothelin-1 (ET-1) in vivo in nine GBE samples was conducted. The analysis results indicated that three bioactive compounds had close relevance to ET-1: Kaempferol-3-O-α-l-glucoside, 3-O-{2-O-{6-O-[P-OH-trans-cinnamoyl]-β-d-glucosyl}-α-rhamnosyl} Quercetin isomers, and 3-O-{2-O-{6-O-[P-OH-trans-cinnamoyl]-β-d-glucosyl}-α-rhamnosyl} Kaempferide. The discovery of bioactive compounds could provide references for the quality control and novel pharmaceuticals development of GRE. The present work proposes a feasible chromatogram-bioactivity correlation based approach to discover the compounds and define their bioactivities for the complex multi-component systems.

Keywords: Ginkgo biloba Extract (GBE); bioactive compounds; chromatogram-bioactivity correlation; endothelial function.

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Conflict of interest statement

All the authors involved in this research have no competing interests in the submission of this manuscript. All the materials were exclusively for our experiments and did not represent any commercial company’s interests. There was no financial support from commercial companies. There are no patents, products in development or marketed products to declare. This information does not alter our adherence to all the Molecules policies on sharing data and materials.

Figures

Figure 1
Figure 1
Research process for discovery of bioactive compounds affecting endothelial function in GBE.
Figure 2
Figure 2
The HPLC fingerprint of GBE with UV (A) and ELSD (B,C).
Figure 3
Figure 3
The HPLC fingerprints of three portions separated from GBE via D101 macroporous resin.
Figure 4
Figure 4
The HPLC fingerprints of nine GBE samples with UV (A) and ELSD (B,C).
Figure 5
Figure 5
The dendrogram of cluster analysis of the nine GBE samples.
Figure 6
Figure 6
Three core bioactive compounds of GBE related to ET-1.
See this image and copyright information in PMC

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