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Comparative Study
. 2018 Oct-Dec;49(4):909-913.
doi: 10.1016/j.bjm.2018.02.011. Epub 2018 Apr 27.

Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan

Anwar Sheed Khan  1 , Sajid Ali  2 , Muhammad Tahir Khan  3 , Sajjad Ahmed  4 , Yasir Khattak  5 , Abduljabbar  6 , Muhammad Irfan  7 , Wasim Sajjad  8
Affiliations
Comparative Study

Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan

Anwar Sheed Khan et al. Braz J Microbiol. 2018 Oct-Dec.

Abstract

GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.

Keywords: GeneXpert; LED-FM microscopy; Sensitivity; Specificity.

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