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Review
. 2018 Apr 5;8(2):17-25.
eCollection 2018.

Application of laser scanning confocal microscopy in the soft tissue exquisite structure for 3D scan

Zhaoqiang Zhang  1 , Mohamed Ibrahim  2 , Yang Fu  3 , Xujia Wu  3 , Fei Ren  4 , Lei Chen  5
Affiliations
Review

Application of laser scanning confocal microscopy in the soft tissue exquisite structure for 3D scan

Zhaoqiang Zhang et al. Int J Burns Trauma. .

Abstract

Three-dimensional (3D) printing is a new developing technology for printing individualized materials swiftly and precisely in the field of biological medicine (especially tissue-engineered materials). Prior to printing, it is necessary to scan the structure of the natural biological tissue, then construct the 3D printing digital model through optimizing the scanned data. By searching the literatures, magazines at home and abroad, this article reviewed the current status, main processes and matters needing attention of confocal laser scanning microscope (LSCM) in the application of soft tissue fine structure 3D scanning, empathizing the significance of LSCM in this field.

Keywords: 3D printing; LSCM; tissue engineering.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
After the illuminant passes through the pinhole, only punctiform illuminant emits and focuses on an object via the lens so as to reach a precise point-to-point effect to scan on plane x-y forming an image of the plane. Deeper scanning can result in more images and the 3D structure drawing can be constructed by computer processing.
Figure 2
Figure 2
We used GFP to label type I collagen and observed forms of type I collagen in tissue sections via 3D reconstruction. A. Is an image under a 200× field where the green fluorescence indicated existence of collagen fibers. B. Is a 3D image reconstructed by ZEN software under a 200× field after 27 optical sections by scanning.
See this image and copyright information in PMC

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