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Review
. 2018 Apr 3:2018:8947548.
doi: 10.1155/2018/8947548. eCollection 2018.

Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure

Sam L Francis  1   2 , Serena Duchi  1   2 , Carmine Onofrillo  1   2 , Claudia Di Bella  1   2 , Peter F M Choong  1   2
Affiliations
Review

Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure

Sam L Francis et al. Stem Cells Int. .

Abstract

Mesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar fat pad (IFP) is a promising source of ADMSCs with respect to producing a cartilage lineage. Cell isolation protocols to date are time-consuming and follow conservative approaches that rely on a long incubation period of 24-48 hours. The different types of ADMSC isolation techniques used for cartilage repair will be reviewed and compared with the view of developing a rapid one-step isolation protocol that can be applied in the context of a surgical procedure.

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Figures

Figure 1
Figure 1
(Modified and used with permission from Wiley under CC BL). Infrapatellar fat pad (IFP) location and harvested tissue. (a) Sagittal magnetic resonance imaging scan of the knee showing the relationship of the IFP (arrow) to the articular cartilage (double arrow). (b, c) Excised IFP from a patient undergoing knee arthroplasty (b) has the fat removed from the fibrous tissue (c). (d, e) The arthroscopically harvested fat pad (d) was separated from the irrigation fluid before enzymatic digestion (e).
Figure 2
Figure 2
Adipose-derived mesenchymal stem cell (ADMSC) isolation protocol including timeframes.
Figure 3
Figure 3
Plastic adherence and morphology of mesenchymal stem cells isolated from the infrapatellar fat pad, representative view using bright field microscopy.
Figure 4
Figure 4
Proposed rapid adipose-derived mesenchymal stem cell (ADMSC) isolation procedure from the infrapatellar fat pad (IFP). The three major changes proposed are highlighted in red.
See this image and copyright information in PMC

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