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. 2015 Sep;1(3):152-159.
doi: 10.1016/j.aninu.2015.08.009. Epub 2015 Sep 1.

Dietary energy intake affects fetal survival and development during early and middle pregnancy in Large White and Meishan gilts

Long Che  1   2 , Zhenguo Yang  1   2 , Mengmeng Xu  1   2 , Ziyun Zhang  1   2 , Peilin Liu  1   2 , Shengyu Xu  1   2 , Lianqiang Che  1   2 , Yan Lin  1   2 , Zhengfeng Fang  1   2 , Bin Feng  1   2 , Jian Li  1   2 , De Wu  1   2
Affiliations

Dietary energy intake affects fetal survival and development during early and middle pregnancy in Large White and Meishan gilts

Long Che et al. Anim Nutr. 2015 Sep.

Abstract

This experiment was designed to determine the effects of variations in dietary energy intake on reproductive performance and gene expression of luteal and endometrium tissues in Large White (LW) and Meishan (MS) gilts during early and middle pregnancy. After insemination, 32 LW gilts were assigned to high and low (HEL and LEL, 14.23 and 12.56 MJ DE/kg, respectively) diet treatment groups, while 32 MS gilts were allocated to HEM and LEM (12.56 and 10.88 MJ DE/kg) groups. Gilts were slaughtered on days 35, 55 and 90 of gestation. The fetal survival and luteal progesterone (P4) concentration in the HEL group were higher on day 35 but lower on day 90 of gestation compared with the LEL group (P < 0.05) for LW gilts. However, fetal survival and luteal P4 concentration on day 35 of gestation were greater (P < 0.05) in the LEM group than in the HEM group for MS gilts, but no significant difference in mid-gestation was showed. The fetal weights of both breeds were higher for the high energy diets compared with the respective control group on day 90 of gestation (P < 0.05). In addition, the mRNA levels of P4 synthesis-related proteins had correlated with luteal P4 concentration in both breeds. Further, endometrial levels of uteroferrin (ACP5), retinol-binding protein 4 (RBP4) and secreted phosphoprotein 1 (SPP1) mRNA were upregulated in the HEL group on day 35 of gestation but ACP5 and SPP1 were downregulated on day 55 of gestation compared with the LEL group (P < 0.05) for LW gilts. In MS gilts, diet only affected the expression of SPP1 (P < 0.05). Our results revealed the differential sensitivity of LW and MS breeds to variations in dietary energy intake. For LW gilts, the HEL group improved fetal survival on day 35 but a sustained high energy diet decreased fetal survival on day 90 of gestation. The differences in dietary energy intake did not influence fetal survival on day 90 of gestation but the higher energy diet did increase fetal weight in the MS breed compared with the lower energy intake diet. These results may be due to differential luteal secretion activity and endometrium gene expression in these two breeds.

Keywords: Corpus luteum; Endometrium; Energy level; Fetal survival; pig.

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Figures

Fig. 1
Fig. 1
Relative mRNA expressions of SR-BI, LDLR, STAR, CYP11A1 and 3β-HSD in luteal from different energy intake on days 35, 55 and 90 of gestation in Large White gilts. Energy levels were varied by supplementing soybean oil and they were 14.23 and 12.56 MJ DE/kg (HEL and LEL) for LW gilts.a,bbars with different letters are significantly different at P < 0.05. SR-BI = scavenger receptor-BI; LDLR = low-density lipoprotein receptor; STAR = steroidogenic acute regulatory protein; CYP11A1 = cytochrome P450scc; 3β-HSD = 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase.
Fig. 2
Fig. 2
Relative mRNA expressions of SR-BI, LDLR, STAR, CYP11A1 and 3β-HSD in luteal from different energy intake on days 35, 55 and 90 of gestation in Meishan gilts. Energy levels were varied by supplementing soybean oil and they were 12.56 and 10.88 MJ DE/kg (HEM and LEM) for MS gilts.a,bBars with different letters are significantly different at P < 0.05. SR-BI = scavenger receptor-BI; LDLR = low-density lipoprotein receptor; STAR = steroidogenic acute regulatory protein; CYP11A1 = cytochrome P450scc; 3β-HSD = 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase.
Fig. 3
Fig. 3
Relative mRNA expressions of PGR, ACP5, SPP1, FGFR2 and RBP4 in endometrium from different energy intake on days 35, 55 and 90 of gestation in Large White gilts. Energy levels were varied by supplementing soybean oil and they were 14.23 and 12.56 MJ DE/kg (HEL and LEL) for LW gilts. a,bBars with different letters are significantly different at P < 0.05. PGR = progesterone receptor; ACP5 = uteroferrin; SPP1 = secreted phospoprotein 1; FGFR2 = fibroblast growth factor receptor 2; RBP4 = retinol-binding protein 4.
Fig. 4
Fig. 4
Relative mRNA expressions of PGR, ACP5, SPP1, FGFR2 and RBP4 in endometrium from different energy intake on days 35, 55 and 90 of gestation in Meishan gilts. Energy levels were varied by supplementing soybean oil and they were 14.23 and 12.56 MJ DE/kg (HEL and LEL) for LW gilts. a,bBars with different letters are significantly different at P < 0.05. PGR = progesterone receptor; ACP5 = uteroferrin; SPP1 = secreted phospoprotein 1; FGFR2 = fibroblast growth factor receptor 2; RBP4 = retinol-binding protein 4.
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