Conditional Gene-Targeting in Mice: Problems and Solutions

Affiliations

¹ Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland. Electronic address: becher@immunology.uzh.ch.
² Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, 55131 Mainz, Germany.
³ Division of Biological Sciences, Center for Microbiome Innovation and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.

Burkhard Becher et al. Immunity. 2018.

. 2018 May 15;48(5):835-836.

doi: 10.1016/j.immuni.2018.05.002.

¹ Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland. Electronic address: becher@immunology.uzh.ch.
² Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Str. 67, 55131 Mainz, Germany.
³ Division of Biological Sciences, Center for Microbiome Innovation and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.

No abstract available

References

1. Croxford AL, Lanzinger M, Hartmann FJ, Schreiner B, Mair F, Pelczar P, Clausen BE, Jung S, Greter M, and Becher B (2015). The Cytokine GM-CSF Drives the Inflammatory Signature of CCR2+ Monocytes and Licenses Autoimmunity. Immunity 43, 502–514. - PubMed
1. Gong S, Doughty M, Harbaugh CR, Cummins A, Hatten ME, Heintz N, and Gerfen CR (2007). Targeting Cre recombinase to specific neuron populations with bacterial artificial dconstructs. J. Neurosci 27, 9817–9823. - PMC - PubMed
1. Gu H, Marth JD, Orban PC, Mossmann H, and Rajewsky K (1994). Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265, 103–106. - PubMed
1. Heffner CS, Herbert Pratt C, Babiuk RP, Sharma Y, Rockwood SF, Donahue LR, Eppig JT, and Murray SA (2012). Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource. Nat. Commun 3, 1218. - PMC - PubMed
1. Klein U, Casola S, Cattoretti G, Shen Q, Lia M, Mo T, Ludwig T, Rajewsky K, and Dalla-Favera R (2006). Transcription factor IRF4 controls plasma cell differentiation and class-switch recombination. Nat. Immunol 7, 773–782. - PubMed