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. 1988 Oct;214(2):313-6.
doi: 10.1007/BF00337727.

Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli

E P Efimova  1 , E P Delver, A A Belogurov
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Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli

E P Efimova et al. Mol Gen Genet. 1988 Oct.

Abstract

The host-controlled EcoK-restriction of unmodified phage lambda.O is alleviated in dam mutants of Escherichia coli by 100- to 300-fold. In addition, the EcoK modification activity is substantially decreased in dam- strains. We show that type I restriction (EcoB, EcoD and EcoK) is detectably alleviated in dam mutants. However, no relief of EcoRI restriction (Type II) occurs in dam- strains and only a slight effect of dam mutation on EcoP1 restriction (Type III) is observed. We interpret the alleviation of the type I restriction in dam- strains to be a consequence of induction of the function which interferes with type I restriction systems.

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