Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Mar 26:2018:6039453.
doi: 10.1155/2018/6039453. eCollection 2018.

Blood and Tissue Enzymatic Activities of GDH and LDH, Index of Glutathione, and Oxidative Stress among Breast Cancer Patients Attending Referral Hospitals of Addis Ababa, Ethiopia: Hospital-Based Comparative Cross-Sectional Study

Mohammed Mehdi  1 , M K C Menon  1 , Nebiyou Seyoum  2 , Mahteme Bekele  3 , Wondimagegn Tigeneh  4 , Daniel Seifu  1
Affiliations

Blood and Tissue Enzymatic Activities of GDH and LDH, Index of Glutathione, and Oxidative Stress among Breast Cancer Patients Attending Referral Hospitals of Addis Ababa, Ethiopia: Hospital-Based Comparative Cross-Sectional Study

Mohammed Mehdi et al. Oxid Med Cell Longev. .

Abstract

The exact cause of breast cancer is unknown; it is a multifactorial disease. It is the most diagnosed and the second killer cancer among women. Breast cancer can be originated from tissues of breast or secondary from other organs via metastasis. Generally, cancer cells show aberrant metabolism and oxidative stress when compared to noncancerous tissues of breast cancer patients. The current study aims at evaluating glutamate and glucose metabolism through GDH and LDH enzyme activities, oxidant, and antioxidative status among breast cancer patients attending referral hospitals of Addis Ababa, Ethiopia. Result. Catalytic activities of glutamate dehydrogenase, lactate dehydrogenase, and oxidative stress index were significantly increased in both serum (4.2 mU/ml, 78.6 mU/ml, and 3.3 : 1, resp.) and cancerous tissues (1.4 mU/ml, 111.7 mU/ml, and 2.15 : 1, resp.) of breast cancer patients as compared to those in serum of control group (3.15 mU/ml, 30.4 mU/ml, and 2.05 : 1, resp.) and noncancerous tissues of breast cancer patients (0.92 mU/ml, 70.5 mU/ml, and 1.1 : 1, resp.) (P ≤ 0.05). Correspondingly, ratios of reduced to oxidized glutathione were significantly decreased in both serum (20 : 1) and cancerous tissues (23.5 : 1) of breast cancer patients when compared to those in serum of control group (104.5 : 1) and noncancerous tissues of breast cancer patients (70.9 : 1) (P ≤ 0.05). Conclusion. Catalytic activities of GDH and LDH, ratios of GSH to GSSG, and concentration of TOS among breast cancer patients were significantly higher than were those among control group and noncancerous tissues of breast cancer patients, while TAC of breast cancer patients is significantly lower than that of control group and normal tissues of breast cancer patients.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Chart showing the workflow of sample processing, Addis Ababa, Ethiopia, 2015–2017.
Figure 2
Figure 2
A bar graph showing glutathione index in serum and cancerous tissues of breast cancer (BCA) patients in comparison to serum of control group and noncancerous tissues of breast cancer patients, Addis Ababa, Ethiopia, 2015–2017.
Figure 3
Figure 3
A bar graph showing oxidative stress index in serum and cancerous tissues of breast cancer (BCA) patients in comparison to serum of control group and noncancerous tissue of breast cancer patients, Addis Ababa, Ethiopia, 2015–2017.
Figure 4
Figure 4
Mean plots of oxidative stress index in serum and tumor tissue samples of breast cancer patients, Addis Ababa, Ethiopia, 2015–2017.
See this image and copyright information in PMC

References

    1. Fard A. E., Zarepour A., Zarrabi A., Shanei A., Salehi H. Synergistic effect of the combination of triethylene-glycol modified Fe3O4 nanoparticles and ultrasound wave on MCF-7 cells. Journal of Magnetism and Magnetic Materials. 2015;394:44–49. doi: 10.1016/j.jmmm.2015.06.040. - DOI
    1. Genetic home reference. Breast cancer. 2015. October 2015, http://ghr.nlm.nih.gov/glossary=malignancy.
    1. American Cancer Society. Cancer Facts & Figures 2017. Atlanta: American Cancer Society; 2017.
    1. Koukourakis M. I., Giatromanolaki A., Harris A. L., Sivridis E. Comparison of metabolic pathways between cancer cells and stromal cells in colorectal carcinomas: a metabolic survival role for tumor-associated stroma. Cancer Research. 2006;66(2):632–637. doi: 10.1158/0008-5472.CAN-05-3260. - DOI - PubMed
    1. Mazure N. M. Hypoxia signalling in cancer and approaches to enforce tumour regression. Nature. 2006;441(7092):437–443. doi: 10.1038/nature04871. - DOI - PubMed