Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Dec;14(6):545-51.
doi: 10.1007/BF00434079.

Isolation and characterization of a Neurospora glucose-repressible gene

M T McNally  1 , S J Free
Affiliations

Isolation and characterization of a Neurospora glucose-repressible gene

M T McNally et al. Curr Genet. 1988 Dec.

Abstract

Using differential hybridization, the cDNA copy of a Neurospora gene coding for an abundant glucose-repressible mRNA (grg-1) has been isolated. The cDNA was used to clone the genomic copy, and both were sequenced. The cDNA is nearly full length and contains putative translational start and termination codons. Conceptual translation indicates that grg-1 mRNA could direct the synthesis of a 7,000 molecular weight polypeptide. The genomic clone, contained in an 1,888 bp PvuII fragment, encompasses the entire cDNA as well as 838 bp of 5' and 369 bp of 3' flanking sequence. Comparison of the cDNA and genomic clones revealed the presence of two short introns in potential protein-coding sequences. grg-1 message levels were found to increase within minutes following the onset of glucose deprivation and rise 50 fold during the first 90 min of derepression.

PubMed Disclaimer

References

    1. Plasmid. 1985 Jan;13(1):31-40 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Mol Cell Biol. 1987 Jul;7(7):2477-83 - PubMed
    1. Mol Cell Biol. 1986 Jul;6(7):2452-61 - PubMed
    1. Nucleic Acids Res. 1987 Feb 25;15(4):1353-61 - PubMed

Publication types

MeSH terms

LinkOut - more resources