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. 2018 Jun;8(6):565-570.
doi: 10.3892/br.2018.1090. Epub 2018 Apr 23.

Evaluation of attenuative effect of tert-butylhydroquinone against diazinon-induced oxidative stress on hematological indices in male Wistar rats

Saman Sargazi  1   2 , Hamidreza Galavi  2 , Sadegh Zarei  3
Affiliations

Evaluation of attenuative effect of tert-butylhydroquinone against diazinon-induced oxidative stress on hematological indices in male Wistar rats

Saman Sargazi et al. Biomed Rep. 2018 Jun.

Abstract

Diazinon is an organophosphate toxicant that has been identified to induce oxidative stress within biological systems through altering biochemical and hematological indices, by generating free radicals while deteriorating the cellular antioxidant defense system. The present study was designed to evaluate the effectiveness of tert-butylhydroquinone (TBHQ), a synthetic food additive, in serving as an antioxidant against sub-lethal diazinon-induced oxidative stress in male Wistar rats. Animals were randomly divided into 4 groups: Control (treated with corn oil as a vehicle), diazinon (10 mg/kg/day), TBHQ (0.028 g/kg of diet) and a combination group of TBHQ (0.028 g/kg of diet) plus diazinon (10 mg/kg/day). All animals were treated orally once a day by gastric tube and treatments were continued for 7 weeks. Hematological indices, including red blood cell (RBC) indices and white blood cell (WBC) and platelet counts, were measured at the end of the treatment schedule. On comparison of the treatment groups with the untreated control group, RBC count, hemoglobin (Hb), hematocrit (Hct), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were significantly decreased in the diazinon and TBHQ+diazinon groups, while MCHC in the TBHQ group and MCV in the diazinon and TBHQ+diazinon groups were significantly increased. When the diazinon and TBHQ+diazinon groups were compared with the TBHQ group, RBC, Hb, Hct, and MCHC were significantly decreased whereas MCV was increased in both groups. Additionally, when comparing the TBHQ+diazinon group with the diazinon group, MCHC was determined to be significantly decreased. The results of the current experiments suggested that TBHQ could not efficiently protect blood cells against diazinon toxicity.

Keywords: diazinon; organophosphate; pesticide; tert-butylhydroquinone.

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Figures

Figure 1.
Figure 1.
Mean values of WBC count in control, TBHQ and/or diazinon-treated rats. WBC, white blood cell; TBHQ, tert-butylhydroquinone.
Figure 2.
Figure 2.
Mean values of RBC, Hb and Hct in control, TBHQ and/or diazinon-treated rats. ***P<0.001 vs. control; ###P<0.001 vs. TBHQ. RBC, red blood cell; Hb, hemoglobin; Hct, hematocrit; TBHQ, tert-butylhydroquinone.
Figure 3.
Figure 3.
Mean values of MCV, MCH and MCHC in control, TBHQ and/or diazinon-treated rats. *P<0.05, ***P<0.001 vs. control; #P<0.05, ###P<0.001 vs. TBHQ; $P<0.05 vs. Diazinon. MCV, mean cell volume; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; TBHQ, tert-butylhydroquinone.
Figure 4.
Figure 4.
Mean values of platelet count in control, TBHQ and/or diazinon-treated rats. TBHQ, tert-butylhydroquinone.
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