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. 2018 May 21;11(1):330.
doi: 10.1186/s13104-018-3438-y.

Effects of ionizing radiation on the viability and proliferative behavior of the human glioblastoma T98G cell line

Hossam Murad  1 , Yaman Alghamian  2 , Abdulmunim Aljapawe  3 , Ammar Madania  4
Affiliations

Effects of ionizing radiation on the viability and proliferative behavior of the human glioblastoma T98G cell line

Hossam Murad et al. BMC Res Notes. .

Abstract

Objective: Radiotherapy is the traditional therapy for glioma patients. Glioma has poor response to ionizing radiation (IR). Studying radiation-induced cell death can help in understanding the cellular mechanisms underlying its radioresistance. T98G cell line was irradiated with Co60 source by 2 or 10 Gy. MTT assay was used to calculate the surviving fraction. Cell viability, cell cycle distribution and apoptosis assays were conducted by flow cytometry for irradiated and control cells for the 10 Gy dose.

Results: The SF2 value for irradiated cells was 0.8. Cell viability was decreased from 93.29 to 73.61%, while, the Sub G0/G1 phase fraction was significantly increased at 10 Gy after 48 h. On the other hand, there was an increase in the percentage of apoptotic cells which reached 40.16% after 72 h at the same dose, while, it did not exceeds 2% for non-irradiated cells. Our results showed that, the T98G cells is radioresistant to IR up to 10 Gy. Effects of irradiation on the viability of T98G cells were relatively mild, since entering apoptosis was delayed for about 3 days after irradiation.

Keywords: Apoptosis; Flow cytometry; Glioma; Ionizing radiation; MTT; Radioresistance; T98G cell line.

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Figures

Fig. 1
Fig. 1
Determination of the radiosensitivity of the T98G cell line using the MTT method. Absorbance values were converted to cells number using a logarithmic line equation of a stander curve for each point, Y axis: cell number, X axis: time. Irradiation of T98G cells with a 2 Gy dose caused a growth delay of about 12 h compared to non-irradiated cells (control). The experiment has been repeated three times and data are expressed as the mean ± SD
Fig. 2
Fig. 2
a Effect of irradiation with a 10 Gy dose on the viability of T98G cell line. Flow cytometry histogram showing the changes in percentage of dead (colored by PI, in red) and live cells (colored by TO and PI, in green), with elapsed time after irradiation indicated. b Effect of irradiation with a 10 Gy dose on T98G cell cycle distribution. Flow cytometry histogram showing the cell distribution according to DNA content
Fig. 3
Fig. 3
Effect of irradiation with a 10 Gy dose in inducing apoptosis in the T98G cell line. Shown is the percentage of early apoptosis cells (annexin V+ PI–, R3 quadrant) and late apoptosis cells (annexin V+ PI+, R1 quadrant) at 24, 48, 72 h after irradiation
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