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Meta-Analysis
. 2018 Jul;35(7):1247-1257.
doi: 10.1007/s10815-018-1206-5. Epub 2018 May 21.

Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia: a systematic review and meta-analysis

Zhe Yu  1   2 , Zhewen Wei  1   2 , Jun Yang  3   4 , Tao Wang  1   2 , Hongyang Jiang  1   2 , Hao Li  1   2 , Zhe Tang  1   2 , Shaogang Wang  1   2 , Jihong Liu  5   6
Affiliations
Meta-Analysis

Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia: a systematic review and meta-analysis

Zhe Yu et al. J Assist Reprod Genet. 2018 Jul.

Abstract

Purpose: The purpose of the study is to explore testicular sperm cryopreservation in patients with nonobstructive azoospermia (NOA) whether affect the outcome of subsequent intracytoplasmic sperm injection (ICSI).

Methods: A systematic review and meta-analysis was conducted by searching the MEDLINE and EMBASE databases for relevant published studies in English language (1997-2017). Studies were eligible if they included the comparison of using fresh and frozen-thawed testicular sperm followed by ICSI. Two reviewers independently performed data extraction, quality assessment and assessed the risk of bias. The overall summary risk estimated the number of events. A meta-analysis was conducted using a random effects or fixed effects model analysis according to the test of heterogeneity.

Results: A total of 17 studies with 1,261 ICSI cycles were identified. Analysis of the present data showed no difference in the fertilization outcome when comparing fresh versus frozen-thawed spermatozoa (RR = 1.02, 95% CI 0.86-1.09). Similarly, no difference in CR (RR = 1.01, 95% CI 0.96-1.05), good embryo rate (RR = 1.01, 95% CI 0.95-1.09), and IR (RR = 0.93, 95% CI 0.66-1.30) was observed if the spermatozoa was fresh or frozen-thawed. Finally, no difference in CPR or LBR was noted when using fresh or frozen-thawed cycles were analyzed separately (RR = 1.03, 95% CI 0.86-1.24; RR 1.11, 95% CI 0.88-1.41, respectively).

Conclusions: In men with NOA, the ICSI outcome is not affected by whether the retrieved testicular sperm is fresh or frozen. Sperm cryopreservation ought to be considered in every surgical sperm retrieval case, which remain feasible even in patients with few testicular sperm retrieved.

Keywords: Cryopreservation; Intracytoplasmic sperm injection; Nonobstructive azoospermia; Testicular sperm extraction.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Flowchart of included studies
Fig. 2
Fig. 2
Egger’s adjusted rank correlation test
Fig. 3
Fig. 3
Forest plot of summary risk estimates for fertilization rate (FR). RR = relative risk; CI = confidence interval
Fig. 4
Fig. 4
Meta-analysis of ICSI outcome using fresh or frozen-thawed testicular sperm in men with NOA. a Cleavage rate (CR), b good embryo rate, and c implantation rate (IR). RR = relative risk; CI = confidence interval
Fig. 5
Fig. 5
Meta-analysis of ICSI outcome using fresh or frozen-thawed testicular sperm in men with NOA. a Clinical pregnancies rate (CPR) and b live birth rate (LBR) per ICSI cycle. RR = relative risk; CI = confidence interval
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