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Review

Murine GLUTag Cells

Manuel Gil-Lozano  1 , Patricia L. Brubaker  1   2
Kitty Verhoeckx  1 , Paul Cotter  2 , Iván López-Expósito  3 , Charlotte Kleiveland  4 , Tor Lea  4 , Alan Mackie  5 , Teresa Requena  6 , Dominika Swiatecka  7 , Harry Wichers  8
, editors.
In: The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet]. Cham (CH): Springer; 2015. Chapter 21.
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Review

Murine GLUTag Cells

Manuel Gil-Lozano et al.
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Excerpt

The incretin hormone, glucagon-like peptide-1 (GLP-1) serves as a link between ingested nutrients and insulin secretion. Because of the anti-diabetic properties exerted by GLP-1, several derivative drugs are currently in the market for the treatment of patients with type 2 diabetes. Over the past few years, several cell lines have been established as useful models for the study of the GLP-1-secreting enteroendocrine L-cells. This review focuses on the murine GLUTag cell line, derived from colonic tumours of transgenic mice expressing large T antigen under the control of the proglucagon promoter. These cells are widely used to examine the effects of food components on GLP-1 secretion, as well as the signaling pathways underlying nutrient-induced GLP-1 release. The effects of different food components, as well as of nutrient-related signals, on GLP-1 secretion, and the protocols for the maintenance and use of the murine GLUTag L-cell line will be discussed.

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References

    1. Anini Y, Brubaker PL (2003) Role of leptin in the regulation of glucagon-like peptide-1 secretion. Diabetes 52:252–259 - PubMed
    1. Brubaker PL, Schloos J, Drucker DJ (1998) Regulation of glucagon-like peptide-1 synthesis and secretion in the GLUTag enteroendocrine cell line. Endocrinology 139:4108–4114 - PubMed
    1. Chu ZL, Carroll C, Alfonso J et al (2008) A role for intestinal endocrine cell-expressed g protein-coupled receptor 119 in glycemic control by enhancing glucagon-like peptide-1 and glucose-dependent insulinotropic peptide release. Endocrinology 149:2038–2047 - PubMed
    1. Dong CX, Brubaker PL (2012) Ghrelin, the proglucagon-derived peptides and peptide YY in nutrient homeostasis. Nat Rev Gastroenterol Hepatol 9:705–715 - PubMed
    1. Drucker DJ, Jin T, Asa SL et al (1994) Activation of proglucagon gene transcription by protein kinase-A in a novel mouse enteroendocrine cell line. Mol Endocrinol 8:1646–1655 - PubMed

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