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Review

PBMC-Derived T Cells

Daniel Lozano-Ojalvo  1 , Rosina López-Fandiño  1 , Iván López-Expósito  1
Kitty Verhoeckx  1 , Paul Cotter  2 , Iván López-Expósito  3 , Charlotte Kleiveland  4 , Tor Lea  4 , Alan Mackie  5 , Teresa Requena  6 , Dominika Swiatecka  7 , Harry Wichers  8
, editors.
In: The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet]. Cham (CH): Springer; 2015. Chapter 16.
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Review

PBMC-Derived T Cells

Daniel Lozano-Ojalvo et al.
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Excerpt

T cell cultures are a valuable tool in food research to perform studies within the food allergy field. Their main applications aim to analyze immunological responses towards food protein antigens to gain further insights into the mechanisms responsible for the development of oral tolerance or for the triggering of food allergies. This chapter describes the main applications, isolation techniques, and culture conditions for PBMC-derived T cells. Furthermore, critical parameters of the model, together with the experimental read outs will be discussed.

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References

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