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Review

Peripheral Blood Mononuclear Cells

Charlotte R. Kleiveland  1   2
Kitty Verhoeckx  1 , Paul Cotter  2 , Iván López-Expósito  3 , Charlotte Kleiveland  4 , Tor Lea  4 , Alan Mackie  5 , Teresa Requena  6 , Dominika Swiatecka  7 , Harry Wichers  8
, editors.
In: The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet]. Cham (CH): Springer; 2015. Chapter 15.
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Review

Peripheral Blood Mononuclear Cells

Charlotte R. Kleiveland.
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Excerpt

Numerous cell types are involved in maintenance of the intestinal tissue. However, the main players are cells of the epithelial lining and the immune system. Human peripheral blood mononuclear cells (PBMCs) are used to investigate the effect of food bioactives on various immune cells. These cells are easily isolated from blood of healthy donors or buffy coats (leukocyte concentrates, a by-product from hospital Blood Banks in the manufacturing of red blood cell and thrombocyte concentrates from anti-coagulated whole blood). PBMCs have a different composition, phenotype and activation status than cells found in intestinal tissue. However, this is a useful test system for investigation of immune modulatory effects of food bioactive compounds. Methods for the isolation of PBMCs and how they are used to investigate effects of bioactive components are discussed in this chapter.

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References

    1. Akdis M, Palomares O, van de Veen W et al (2012) TH17 and TH22 cells: a confusion of antimicrobial response with tissue inflammation versus protection. J Allergy Clin Immunol 129:1438–1449, quiz 1450–1431 - PubMed
    1. Ashraf MT, Khan RH (2003) Mitogenic lectins. Med Sci Monit 9:RA265–RA269 - PubMed
    1. Ashraf R, Vasiljevic T, Smith SC et al (2014) Effect of cell-surface components and metabolites of lactic acid bacteria and probiotic organisms on cytokine production and induction of CD25 expression in human peripheral mononuclear cells. J Dairy Sci 97:2542–2558 - PubMed
    1. Crotty S (2011) Follicular helper CD4 T cells (TFH). Annu Rev Immunol 29:621–663 - PubMed
    1. de Kivit S, Saeland E, Kraneveld AD et al (2012) Galectin-9 induced by dietary synbiotics is involved in suppression of allergic symptoms in mice and humans. Allergy 67:343–352 - PubMed

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