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Review

Co-culture Caco-2/Immune Cells

Charlotte R. Kleiveland  1   2
Kitty Verhoeckx  1 , Paul Cotter  2 , Iván López-Expósito  3 , Charlotte Kleiveland  4 , Tor Lea  4 , Alan Mackie  5 , Teresa Requena  6 , Dominika Swiatecka  7 , Harry Wichers  8
, editors.
In: The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet]. Cham (CH): Springer; 2015. Chapter 18.
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Review

Co-culture Caco-2/Immune Cells

Charlotte R. Kleiveland.
Free Books & Documents

Excerpt

Numerous cell types are involved in maintenance of the intestinal tissue. However, the main players are cells of the epithelial lining and from the immune system. All these cells are communicating with each other and are strongly influenced by interactions with neighboring cells. Mono-cultivation of cells may provide valuable information as to understanding basic biology, but co-cultivation of more than one type of cells gives an opportunity to investigate effects of intercellular communication. Examples of systems for co-cultivation of epithelial cells and immune cells for the investigation of both direct cell–cell contact and communication with soluble factors will be discussed in this chapter. In addition, co-cultivation systems for differentiation to more specialized epithelial cells as microfold cells (M-cells) will be described.

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References

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