Correction to: Novel smac mimetic APG-1387 elicits ovarian cancer cell killing through TNF-alpha, Ripoptosome and autophagy mediated cell death pathway

Abstract

In the publication of this article [1], there was an error in Figs. 2, 3 and 6.

Fig. 2

Effects of APG-1387 on apoptosis in ovarian cancer. a APG-1387 inhibited the proliferation of SKOV3 cell line. They were treated with the indicated concentrations of APG-1387 for 24, 48, 72 h. Cell viability was determined by the CCK-8 assay. b Morphology of SKOV3 cells exposed to APG-1387(0, 10 nM) photographed under a fluorescence microscope (original magnification× 10). c APG-1387-induced apoptosis in SKOV3 cells was assessed by Hoechst33258 staining. Morphology of SKOV3 cells exposed to APG-1387 at different concentrations photographed under a fluorescence microscope (original magnification × 10). Condensated and fragmented nuclears were the mean ± SEM of 5 randomized areas. P < 0.01. d SKOV3 cells were treated with 10 nM APG-1387 for the indicated times. The cells were stained for phosphorylated H₂AX and DAPI, then were analyzed by fluorescence microscopy (original magnification × 200). γ-H₂AX positive spots were the mean ± SEM of 5 randomized areas. P < 0.01. e, f SKOV3 and OVCAR3 cells were exposed to various concentrations of APG-1387 (0, 10, 30 nM) for 24 h followed cell apoptosis analysis by flow cytometry. g Western blot analysis of caspase-3/PARP SKOV3 cells were treated withAPG-1387 (0, 3, 10, 30, 100, 300 nM) for 24 h. The data shown are representative of three different experiments. h SKOV3 cells were stimulated with APG-1387 for indicated periods of concentrations, caspase activation were tested by caspase activity assay

Fig. 3

APG-1387-induced apoptosis in caspase dependent manner. a Cells with or without addition of Z-VAD-FMK. Morphology of cells exposed to different treatment groups photographed under a fluorescence microscope (original magnification × 10). b APG-1387 was coadministered with or without addition of caspase inhibitor (Z-VAD-FMK). Cell viability was determined by the CCK-8 assay. c Western blot analysis of the effect of APG-1387 with or without addition of Z-VAD-FMK on caspase-3/PARP expression level in SKOV3 cells. d Western blot analysis of the expression levels of IAPs at different concentrations of APG-1387 in SKOV3 cells. e Cells were treated with different time points, and the effect of APG-1387 on IAP family members expression level was determined by western blot. Data represent one of three experiments yielding similar results

Fig. 6

APG-1387 induces autophagy in ovarian cancer cells. a The expression of LC3, Beclin1 and P62 was measured by western blot. Cells were treated with APG-1387(0, 3, 10, 30, 100, 300 nM) for 24 h. b Cells were transfected with GFP-LC3 plasmids, and then maintained in media with or without 3 nM APG-1387 for 24 h. The cells were then stained with DAPI and analyzed by fluorescence microscopy. c Statistical analysis of the percentage of LC3 puncta per cell. Columns, mean (n = 3); bars, SD. *P < 0.01 vs. untreated group. LC3 puncta per cell were quantified. d Cells were transfected with Beclin1 siRNAs. Western blot was used to detect the expression of Beclin1. e Cells were transfected with ATG7 siRNAs. Western blot was used to detect the expression of ATG7. f Cells were transfected with Beclin1 siRNAs. After 24 h treatment with or without 3 nM APG-1387, western blot analysis was performed for indicated proteins. g Cells were transfected with ATG7 siRNAs. After 24 h treatment with or without 3 nM APG-1387, western blot analysis was performed for indicated proteins. h Western blot analysis was performed for indicated proteins in cells transfected with siBeclin-1 and treated with 10 nM APG-1387. i Western blot analysis was performed for indicated proteins in cells transfected with siATG7–1 and treated with 10 nM APG-1387