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. 2018 Mar;56(1):110-116.
doi: 10.17113/ftb.56.01.18.5477.

Production of Pectinase from Bacillus sonorensis MPTD1

Anju Mohandas  1 , Sindhu Raveendran  1 , Binod Parameswaran  1 , Amith Abraham  1 , Raj S R Athira  1   2 , Anil Kuruvilla Mathew  1 , Ashok Pandey  3
Affiliations

Production of Pectinase from Bacillus sonorensis MPTD1

Anju Mohandas et al. Food Technol Biotechnol. 2018 Mar.

Abstract

Seven isolates from spoiled fruits and vegetables were screened for pectinase production using pectin agar plates and the most efficient bacterial strain, MPTD1, was identified as Bacillus sonorensis. Optimisation of various process parameters was done using Plackett-Burman and Box-Behnken designs and it was found that parameters like yeast extract, K2HPO4, incubation time, NaNO3 and KCl have a negative impact on pectinase production. Parameters like pH and MgSO4 and pectin mass fractions have a positive impact on pectinase production. The maximum obtained enzyme activity was 2.43 (μM/mL)/min. This is the first report on pectinase production by Bacillus sonorensis.

Keywords: Bacillus sonorensis; fermentation optimization; pectinase.

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Figures

Fig. 1
Fig. 1
Pectinase-producing microorganisms showing clear zones on pectin agar plates after Gram’s iodine staining: MPTD1 (a), MPTD4 (b) and MPTD6 (c)
Fig. 2
Fig. 2
Phylogenetic tree expressing the relationship between pectinase-producing bacterial strain MPTD1 with reference strains based on the 16S rDNA sequences. Bacillus subtilis is used as an outgroup. Numbers shown in each node are confidence level generated from 1000 bootstraps. Scale bar is nucleotide substitution per sequence position
Fig. 3
Fig. 3
Pareto chart showing significant parameters affecting pectinase activity
Fig. 4
Fig. 4
Surface (a) and contour (b) plots showing the effect of interactions of MgSO4 and pectin mass fractions on pectinase activity
Fig. 5
Fig. 5
Surface (a) and contour (b) plots showing the effect of interactions of pH and pectin mass fraction on enzyme production
Fig. 6
Fig. 6
Surface (a) and contour (b) plots showing the effect of interactions of MgSO4 mass fraction and pH on enzyme production
See this image and copyright information in PMC

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