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. 2018 Mar 15;4(5):FSO298.
doi: 10.4155/fsoa-2017-0121. eCollection 2018 Jun.

Functional variants of TIM-3/HAVCR2 3'UTR in lymphoblastoid cell lines

Feifei Pu  1   1 , Fengxia Chen  2   2 , Zhicai Zhang  3   3 , Jing Feng  1   1 , Ping Xia  1   1
Affiliations

Functional variants of TIM-3/HAVCR2 3'UTR in lymphoblastoid cell lines

Feifei Pu et al. Future Sci OA. .

Abstract

Aim: Variants of TIM-3/HAVCR2 3'UTR miRNA binding sites are significantly associated with cancer; however, roles in post-transcriptional regulation have not been elucidated.

Methods: The regulatory and coding region single nucleotide polymorphisms (SNPs) of TIM-3/HAVCR2 were identified using an online database. Single nucleotide polymorphism Function Prediction was used to predict potential functional relevance of miRNA binding sites.

Results: The analysis indicated rs9313439, rs4704846, rs3087616 and rs1036199 affect possible miRNA binding sites in TIM-3/HAVCR2 3'UTR. We used additional data on genotypes and limited minor allele frequency >5% in the HapMap populations. Only rs3087616 and rs4704846 were significantly associated with TIM-3/HAVCR2.

Conclusion: Both rs3087616 and rs4704846 could be putative variants mediating post-transcriptional regulation of the TIM-3/HAVCR2. Deeper understanding of how 3'UTR variants influence the activity by TIM-3/HAVCR2 for therapy against cancer.

Keywords: TIM-3/HAVCR2; genetic; miRNA; polymorphism; variant.

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Conflict of interest statement

Financial & competing interests disclosure This work is supported by Grants from the Clinical Medical Research Project of Wuhan (number WX17Q38) and the Funded Research Project of Wuhan No.1 Hospital, Wuhan Integrated TCM & Western Medicine Hospital (number 2017Y01). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Figures

<b>Figure 1.</b>
Figure 1.. A linkage disequilibrium plot of the TIM-3/HAVCR2 region generated using the single nucleotide polymorphism Function Prediction software (FuncPred).
Each square number represents the pairwise r2 correlations between the two relevant SNPs. The color of each SNP spot reflects its D′ value, which changes from red to white as the D′ value decreases. MAF: Minor allele frequency; SNP: Single nucleotide polymorphism.
See this image and copyright information in PMC

References

    1. Pharoah PD, Dunning AM, Ponder BA, Easton DF. Association studies for finding cancer-susceptibility genetic variants. 2004;4(11):850–860. - PubMed
    1. Zeljic K, Supic G, Jovic N, et al. Association of TLR2, TLR3, TLR4 and CD14 genes polymorphism with oral cancer risk and survival. 2014;20(4):416–424. - PubMed
    1. Serra M, Hattinger CM. Polymorphisms of genes related to methotrexate response and toxicity in high-grade osteosarcoma. 2017;13(1):123. - PubMed
    1. Du W, Yang M, Turner A, et al. TIM-3 as a target for cancer immunotherapy and mechanisms of action. 2017;18(3) pii:E645. - PMC - PubMed
    1. Gorman JV, Colgan JD. Regulation of T cell responses by the receptor molecule Tim-3. 2014;59(1–3):56–65. - PMC - PubMed

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