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. 1985 Mar;161(3):1219-21.
doi: 10.1128/jb.161.3.1219-1221.1985.

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

S C Winans, S J Elledge, J H Krueger, G C Walker

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

S C Winans et al. J Bacteriol. 1985 Mar.

Abstract

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.

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References

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