doi: 10.1073/pnas.82.3.694.
The herpes simplex virus amplicon: analyses of cis-acting replication functions
- PMID: 2983310
- PMCID: PMC397112
- DOI: 10.1073/pnas.82.3.694
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The herpes simplex virus amplicon: analyses of cis-acting replication functions
Proc Natl Acad Sci U S A.
1985 Feb.
Abstract
Previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. The present study established that two separate cis-acting functions--a DNA replication origin and a cleavage/packaging signal--are required for amplicon propagation. Using deleted derivatives of cloned amplicons, we mapped one of the viral DNA replication origins (ori-2 or oriL) at coordinate 0.422 of the standard HSV-1 genome and at an equivalent position within the HSV-2 genome.
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