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. 1985 Feb;82(3):694-8.
doi: 10.1073/pnas.82.3.694.

The herpes simplex virus amplicon: analyses of cis-acting replication functions

R R Spaete, N Frenkel

The herpes simplex virus amplicon: analyses of cis-acting replication functions

R R Spaete et al. Proc Natl Acad Sci U S A. 1985 Feb.

Abstract

Previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. The present study established that two separate cis-acting functions--a DNA replication origin and a cleavage/packaging signal--are required for amplicon propagation. Using deleted derivatives of cloned amplicons, we mapped one of the viral DNA replication origins (ori-2 or oriL) at coordinate 0.422 of the standard HSV-1 genome and at an equivalent position within the HSV-2 genome.

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