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. 1985 Jan 15;225(2):529-33.
doi: 10.1042/bj2250529.

Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells

A J Strain, W A Wallace, A H Wyllie

Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells

A J Strain et al. Biochem J. .

Abstract

Synchronized CV-1 cells were transfected with SV40 (simian virus 40) DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells as compared with asynchronous controls. No difference was observed when carrier DNA was omitted, when cells had progressed through S-phase and into G2-phase, or when the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.

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