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. 1985;239(2):311-5.
doi: 10.1007/BF00218009.

Evidence for an association between calmodulin and membrane patches containing gonadotropin-releasing hormone--receptor complexes in cultured gonadotropes

L Jennes, D Bronson, W E Stumpf, P M Conn

Evidence for an association between calmodulin and membrane patches containing gonadotropin-releasing hormone--receptor complexes in cultured gonadotropes

L Jennes et al. Cell Tissue Res. 1985.

Abstract

participation of calmodulin, clathrin, and actin in receptor mediated endocytosis of gonadotropin-releasing hormone (GnRH) was studied in an in vitro system of dispersed pituitary cells with a triple staining procedure. Cells were incubated in D-Lys6-Pro9-Des10-GnRH-biotin and stained with avidin-peroxidase-diaminobenzidine. Calmodulin, clathrin, and actin as well as luteinizing hormone were identified by indirect immunofluorescence with FITC- and rhodamine-labeled second antibody. The results indicate a close spatial association of calmodulin, but not of clathrin and actin, with GnRH-containing plasma membrane patches.

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