Characterization of restriction-modification enzymes Cfr13 I from Citrobacter freundii RFL13

Abstract

This communicatiopn describes some properties of RCfr13 I and MCfr13 I, isolated from Citrobacter freundii RFL13. RCFfr13 I restriction enzyme recognizes the 5'-G GNCC sequence and cleaves, as indicated by the arrow. MCfr13 I methylase modifies the internal cytosine producing m5C (5'-GGNm5CC). RCfr13 I is sensitive not only to this type of substrate modification but also to hemimethylation in overlapping sites by MCfr10 I (internal cytosine of RCfr13 I recognition is methylated) and MHpa II (external cytosine is methylated). From these results the sensitivity of RCfr13 I to methylation by dcm methylase of E.coli in overlapping sites is deduced.