Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Mar;21(3):459-61.
doi: 10.1128/jcm.21.3.459-461.1985.

Problems associated with the use of (E)-5-(2-bromovinyl)-2'-deoxyuridine for typing herpes simplex virus

E M Swierkosz, M Q Arens, K A Rivetna

Problems associated with the use of (E)-5-(2-bromovinyl)-2'-deoxyuridine for typing herpes simplex virus

E M Swierkosz et al. J Clin Microbiol. 1985 Mar.

Abstract

When 0.5 microgram of (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) per ml was incorporated directly into cell culture medium inoculated with eight known positive specimens, one herpes simplex virus type 1 (HSV-1) isolate grew in the presence of BVdU and was misidentified. By plaque assay, the titers of 15 HSV-1 strains were reduced by more than 3 log10 by BVdU, and the titers of 16 HSV-2 strains were reduced by less than 2 log10. Titers of HSV-1 acyclovir-resistant strains were reduced by less than 1.5 log10, which was characteristic of HSV-2 strains. Thus, typing of HSV isolates in the presence of BVdU by plaque assay is reliable only if information regarding previous antiviral therapy is obtained.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Biol Chem. 1978 Dec 25;253(24):8721-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 May;78(5):2698-702 - PubMed
    1. Mol Pharmacol. 1981 Jul;20(1):230-3 - PubMed
    1. J Clin Microbiol. 1982 Apr;15(4):733-6 - PubMed
    1. Mol Pharmacol. 1982 Mar;21(2):432-7 - PubMed

MeSH terms

LinkOut - more resources