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. 1985 Jan;5(1):40-5.
doi: 10.1128/mcb.5.1.40-45.1985.

Stable transcription complex on a class III gene in a minichromosome

A B Lassar, D H Hamer, R G Roeder

Stable transcription complex on a class III gene in a minichromosome

A B Lassar et al. Mol Cell Biol. 1985 Jan.

Abstract

We have constructed recombinant simian virus 40 molecules containing Xenopus 5S RNA and tRNA genes. Recombinant minichromosomes containing these genes were isolated to study the interaction of RNA polymerase III transcription factors with these model chromatin templates. Minichromosomes containing a tRNAMet gene can be isolated in a stable complex with transcription factors (IIIB and IIIC) and are active in vitro templates for purified RNA polymerase III. In contrast, minichromosomes containing a 5S RNA gene are refractory to transcription by purified RNA polymerase III in either the absence or the presence of other factors.

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